Which type of cells is compatible with Vascuzell´s seeding technology?

Virtually any cell type can be considered for magnetic cell seeding procedures. We have an extensive experience working with both vascular stem cells and differentiated vascular cells including endothelial cells, smooth muscle cells and fibroblasts from both human and animal origin.

Which type of magnetic nanoparticle is most adequate for magnetic seeding?

Dextran-coated particles have shown excellent results for endothelial and smooth muscle cells labeling. However, many other suitable labeling strategies are commercially available, including magnetic carriers approved for clinical use with a long favorable record.  

Do magnetic nanoparticles alter cell viability or function?

We have assessed the viability and specific metabolic parameters of numerous magnetically labeled cell lines with no adverse results.

Cell labeling protocols require low concentrations of commercially available magnetic nanoparticles. The iron load inside the cells rapidly decreases as a result of nanoparticle inheritance to daughter cells during proliferation. Some carriers have even proved their safety when injected directly into the patient´s bloodstream as MRI contrast agents.

Could the magnetic field alter cells during the seeding process?

During the seeding process cells are exposed to a magnetic field which is about 100 times weaker than the field generated by a regular Magnetic Resonance Imaging (MRI) scanner.

How long does the seeding process take?

The Endothelizer® delivers cells onto the growth surface within seconds. The magnetic field is maintained to allow a consistent adhesion of the cells to the target surface, avoiding subsequent sedimentation due to gravity. Based on our experience, the total seeding process ranges from 15 to 30 minutes depending on the cell type and the target surface.

What types of scaffold geometries are compatible with the Magnetic Seeding Technology?

Magnetic cell seeding has been initially designed to produce tubular and planar cell-coated implants. We are currently developing new designs to address further complex geometries, such as tubular bifurcations and heart valves.

How can I integrate the Endothelizer® into my experimental setup?

The screen of the Endothelizer® intuitively guides you through the different available configurations. The seeding parameters can be personalized to maximize the use of the Endothelizer® and achieve the specific requirements needed in each experiment.
To ensure the traceability of the results, the main parameters of each seeding process are registered and can be easily exported via USB.